| Barcode |
DNA sequence (5’-3’) |
Conditions for PCR amplification |
Literature |
| matK-3FKIM-r matK-1RKIM-f |
CGATCTATTCATTCAATATTTC TCTAGCACACGAAAGTCGAAGT |
94 ℃ – 5 min; (94 ℃ – 1 min, 48 ℃ – 30 s, 72 ℃ – 1 min) 26 cycles; 72 ℃–7 min |
Ki-Joong Kim, as cited in Kuzmina, Johnson, Barron, & Herbert, 2012 |
| psbH trnH |
GTTATGCATGAACGTAATGCTC CGCGCATGGTGGATTCACAATCC |
94 ℃ – 5 min; (94 ℃ – 1 min, 55 ℃ – 1 min, 72 ℃ – 1.5 min) 30 cycles; 72 ℃ – 7 min |
Tate & Simpson, 2003;Sang, Crawford, & Stuessy, 1997 |
| trnL-F ftrnL-F c |
ATTTGAACTGGTGACACGAGCGAAATCGGTAGACGCTACG |
94 ℃ –5 min; (94 ℃ – 1 min, 55 ℃ - 2 min, 72 ℃ –2 min) 35 cycles; 72 ℃ – 10 min. |
Taberlet, Gielly, Pautou, & Bouvet, 1991 |
| S2F S3R |
ATGCGATACTTGGTGTGAATGACGCTTCTCCAGACTACAAT |
94 °C – 5 min; (94 °C – 0.5 min, 56 °C – 30 s, 72 °C – 1 min) 40 cycles; 72 °C – 7 min |
Chen et al., 2010 |
